The smart Trick of PP88 That Nobody is Discussing
The smart Trick of PP88 That Nobody is Discussing
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In a particular embodiment, claimed given effect on stated qualified receiver bacterial mobile generates, instantly or indirectly, a response in reported organism hosting reported focused receiver bacterial mobile.
262、细菌噬菌体可选自长尾噬菌体科(非限制性地比如以下属：cam病毒、lika病毒、r4病毒、acadian病毒、cooper病毒、pg1病毒、pipefish病毒、rosebush病毒、brujita病毒、che9c病毒、hawkeye病毒、plot病毒、jersey病毒、k1g病毒、sp31病毒、lmd1病毒、una4病毒、bongo病毒、rey病毒、butters病毒、charlie病毒、redi病毒、baxter病毒、nymphadora病毒、bignuz病毒、fishburne病毒、phayonce病毒、kp36病毒、rogue1病毒、rtp病毒、t1病毒、tls病毒、ab18病毒、amigo病毒、anatole病毒、andromeda病毒、attis病毒、barnyard病毒、bernal13病毒、biseptima病毒、bron病毒、c2病毒、c5病毒、cba181病毒、cbast病毒、ceci病毒、che8病毒、chi病毒、cjw1病毒、corndog病毒、cronus病毒、d3112病毒、d3病毒、decurro病毒、demosthenes病毒、doucette病毒、e125病毒、eiau病毒、ff47病毒、gaia病毒、giles病毒、gordon病毒、gordtnk病毒、harrison病毒、hk578病毒、hk97病毒、jenst病毒、jwx病毒、kellezio病毒、korra病毒、l5病毒、lambda病毒、laroye病毒、liefie病毒、marvin病毒、mudcat病毒、n15病毒、nonag病毒、np1病毒、omega病毒、p12002病毒、p12024病毒、p23病毒、p70病毒、pa6病毒、pamx74病毒、patience病毒、pbi1病毒、pepy6病毒、pfr1病毒、phic31病毒、phicbk病毒、phieta病毒、phifel病毒、phijl1病毒、pis4a病毒、psa病毒、psimuna病毒、rdjl病毒、rer2病毒、sap6病毒、send513病毒、septima3病毒、seurat病毒、sextaec病毒、sfi11病毒、sfi21dt1病毒、sitara病毒、sk1病毒、slash病毒、smoothie病毒、soups病毒、spbeta病毒、ssp2病毒、t5病毒、tank病毒、tin2病毒、titan病毒、tm4病毒、tp21病毒、tp84病毒、tria病毒、trigintaduo病毒、vegas病毒、vendetta病毒、wbeta病毒、wildcat病毒、wizard病毒、woes病毒、xp10病毒、ydn12病毒和yua病毒)。
215、在限制性位点序列中，n意指核苷酸可为a、c、g或t；b意指核苷酸可为c、g或t；y意指核苷酸可为c或t；w意指核苷酸可为a或t；r意指核苷酸可为a或g；以及d意指a、g或t。
The present inventors confirmed that it is feasible to derive novel conditionally replicative plasmids, specifically based on the primase-helicase and origin of replication from PICIs. These origins might be fairly exceptional in target strains, plus much more advantageously the primase-ori pair might be exceptional for each PICI, substantially cutting down the opportunity of undesired recombination or payload distribute activities.
provided impact and Corresponding Nucleic Acids of fascination inside the modulating technique of the invention, mentioned nucleic acid of interest produces a presented effect on said qualified receiver bacterial mobile, as defined previously mentioned.
In conclusion, conditional origins of replication according to PICIs permit for creation at high titers of large payloads (˜twelve kb) and nuclease-mediated killing of the target strain within the absence of collection and primase protein.
ideally the genetic modification is carried out within the peptides fragment regarded as epitope from the human immune method resulting in a weaker or absence of epitope recognition because of the human immune procedure.
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inside of a preferred embodiment, the genetic modification is in human commensal germs encoding a Ro60 ortholog gene. if possible, the Ro60 protein ensuing within the genetic modification reveals reduce homology with human Ro60 peptide as compared to the first protein. Preferably the genetic 查看更多內容 modification is performed from the DNA sequence equivalent to peptides fragment acknowledged as epitope via the human immune technique leading to a weaker or absence of epitope recognition with the human immune system.
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In a certain embodiment, the topic has presently acquired at the least a single line of treatment method, if possible quite a few traces of procedure, ahead of the administration in the vectors in accordance with the creation, specially a vector packaged into a shipping auto based on the creation, preferably a packaged plasmid or phagemid into a bacterial virus particle according to the creation, or of a pharmaceutical or veterinary composition according to the invention.
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